Nitrogen removal efficiency of some bacterial strains isolated from seawater in Thua Thien Hue, Vietnam
Abstract
Background: Nitrifying bacteria in aquaculture environments are capable of removing toxic nitrogen compounds such as ammonium and nitrite. Using these indigenous microbial resources can improve shrimp production.
Methods: Screening method was used to isolate aerobic strains of nitrifying bacteria. Species identification for these isolates was done by biomolecular method based on 16S rDNA gene sequence. Ammonium, nitrite and nitrate concentrations from the culture were determined by spectrophotometry at the appropriate wavelength. Temperature, pH, dissolved oxygen and salinity were measured by specialized equipment. Formation and development of flocs during shrimp culture were determined based on their volume and weight. A trial of shrimp nursery was carried out on a small scale with 0.5 m3 tanks containing diluted seawater to 16-18‰ salinity at a density of 400 individual/m3 for 24 days on April 2019.
Results: This study isolated two strains of Pseudomonas (BF01 and BF03) and one strain of Cupriavidus oxalaticus BF02 from seawater in Thua Thien Hue province, Vietnam. These bacterial isolates have shown ability to remove nitrogen compounds such as ammonium, nitrite and nitrate in culture medium. Formation and development of flocs were found in trials of shrimp nursery with diluted seawater containing the isolates. Some water quality parameters (temperature, pH, dissolved oxygen, salinity, ammonium and nitrite) were kept at a safe level and juvenile shrimp grown normally during culture.
Conclusion: The observations on the water quality and basic growth parameters of juvenile shrimp in the two treatments, diluted seawater and diluted seawater with commercial microbial products, showed that there were no significant differences between them with p = 0.05. This proves that three isolates have played an important role in shrimp nursery.
Keywords: Cupriavidus oxalaticus; Floc; Litopenaeus vanamei; Nitrifying-denitrifying bacteria; Pseudomonas sp.
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Chen YH, He JG. Effects of environmental stress on shrimp innate immunity and white spot syndrome virus infection. Fish & Shellfish Immunology, (2019); 84: 744-755.
Yun L, Yu Z, Li Y, Luo P, Jiang X, et al. Ammonia nitrogen and nitrite removal by a heterotrophic Sphingomonas sp. strain LPN080 and its potential application in aquaculture. Aquaculture, (2019); 500: 477-484.
Gross A, Abutbul S, Zilberg D. Acute and chronic effects of nitrite on white shrimp, Litopenaeus vannamei, cultured in low-salinity brackish water. Journal of the World Aquaculture Society, (2004); 35: 315-321.
Wang WN, Wang AL, Zhang Y, Li ZH, Wang JX, et al., Effects of nitrite on lethal and immune response of Macrobrachium nipponense. Aquaculture, (2004); 232: 679-686.
Kautsky N, Rönnbäck P, Tedengren M, Troell M. Ecosystem perspectives on management of disease in shrimp pond farming. Aquaculture, (2000): 191: 145-161.
Ferreira NC, Bonetti C, Seifferta WQ. Hydrological and water quality indices as management tools in marine shrimp culture. Aquaculture, (2011); 318: 425-433.
Brown MN, Briones A, Diana J, Raskin L. Ammonia-oxidizing archaea and nitrite-oxidizing nitrospiras in the biofilter of a shrimp recirculating aquaculture system. FEMS Microbiology Ecology, (2013); 83: 17-25.
Bossier P, Ekasari J. Biofloc technology application in aquaculture to support sustainable development goals. Microbial Biotechnology, (2017); 10: 1012-1016.
Santhana Kumar V, Pandey PK, Anand T, Bhuvaneswari GR, Dhinakaran A, et al. Biofloc improves water, effluent quality and growth parameters of Penaeus vannamei in an intensive culture system. Journal of Environmental Management, (2018); 215: 206-215.
Chen Z, Chang Z, Zhang L, Jiang Y, Ge H, et al. Effects of water recirculation rate on the microbial community and water quality in relation to the growth and survival of white shrimp (Litopenaeus vannamei). BMC Microbiology, (2019); 19: 192.
Emerenciano MGC, Martínez-Córdova LR, Martínez-Porchas M, Miranda-Baeza A. Biofloc Technology (BFT): Tool for Water Quality Management in Aquaculture. Chapter 5. In: Water Quality (Tutu H, ed.), (2017); IntechOpen Ltd., London.
Xu WJ, Pan LQ. Enhancement of immune response and antioxidant status of Litopenaeus vannamei juvenile in biofloc-based culture tanks manipulating high C/N ratio of feed input. Aquaculture, (2013); 412-413: 117-124.
Ruan Y, Taherzadeh MJ, Kong D, Lu H, Zhao H, et al. Nitrogen removal performance and metabolic pathways analysis of a novel aerobic denitrifying halotolerant Pseudomonas balearica strain RAD-17. Microorganisms, (2020); 8: 1.
Luo L, Zhao Z, Huang X, Du X, Wang C, et al. Isolation, identification, and optimization of culture conditions of a bioflocculant-producing bacterium Bacillus megaterium SP1 and its application in aquaculture wastewater treatment. BioMed Research International, (2016); 2016: 2758168.
Qiu X, Wang T, Zhong X, Du G, Chen J. Screening and characterization of an aerobic nitrifying-denitrifying bacterium from activated sludge. Biotechnology and Bioprocess Engineering, (2012); 17: 353-360.
Baird RB, Eaton AD, Rice EW. Standard Methods for the Examination of Water and Wastewater. 23rd ed., (2017); American Public Health Association, American Water Works Association, and Water Environment Federation, USA.
Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, (2018); 35: 1547-1549.
Boyd CE. Water Quality Management and Aeration in Shrimp Farming. 2nd ed., (1989); Fisheries and Allied Aquacultures Departmental Series. Alabama Agricultural Experiment Station, USA.
Avnimelech Y. Carbon/nitrogen ratio as a control element in aquaculture systems. Aquaculture, (1999); 176: 227-235.
Wyk PV, Samocha TM, David AD, Lawrence AL, Collins CR. Intensive and super-intensive production of the Pacific White leg (Litopenaeus vannamei) in greenhouse-enclose raceway system. In Book of Abstracts, Aquaculture 2001, Lake Buena Visa, L, 573P.
Kanda J. Determination of ammonium in seawater based on the indophenol reaction with o- phenylphenol (OPP). Water Research, (1995); 29: 2746-2750.
Xu Y, He T, Li Z, Ye Q, Chen Y. Nitrogen removal characteristics of Pseudomonas putida Y-9 capable of heterotrophic nitrification and aerobic denitrification at low temperature. BioMed Research International, (2017); 1429018.
Fitriyanto NA, Winarti A, Imara FA, Erwanto Y, Hayakawa T. Identification and growth characters of nitrifying Pseudomonas sp., LS3K isolated from Odorous region of Poultry farm. Journal of Biological Sciences, (2017); 17: 1-10.
Zhang J, Wu P, Hao B, Yu Z. Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001. Bioresource Technology, (2011); 102: 9866-9869
Prasetyo RA, Pertiwiningrum A, Erwanto Y, Yusiati LM, Fitriyanto NA. The potency of Pseudomonas sp. LS3K as nitrifying bacteria on inorganic medium at various c/n ratios. Asian Journal of Microbiology, Biotechnology, Environmental Sciences, (2019); 21: 257-263.
Zhou M, Ye H, Zhao X. Isolation and characterization of a novel heterotrophic nitrifying and aerobic denitrifying bacterium Pseudomonas stutzeri KTB for bioremediation of wastewater. Biotechnology Bioprocess Engineering, (2014); 19: 231-238.
Bedade DK, Singhal RS. Biodegradation of acrylamide by a novel isolate, Cupriavidus oxalaticus ICTDB921: Identification and characterization of the acrylamidase produced. Bioresource Technology, (2018); 261: 122-132.
Su JF, Xue L, Huang TL, Wang Z, Wang JX. Kinetic analysis of denitrification coupled with Cd(II) removal by Cupriavidus sp. CC1 and its removal mechanism. Research in Microbiology, (2019); 170: 214-221.
Min J, Chen W, Hu X. Biodegradation of 2,6-dibromo-4-nitrophenol by Cupriavidus sp. strain CNP-8: Kinetics, pathway, genetic and biochemical characterization. Journal of Hazardous Materials, (2019); 361: 10-18.
Sun Z, Lv Y, Liu Y, Ren R. Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a novel metal resistant bacterium Cupriavidus sp. S1. Bioresource Technology, (2016); 220: 142-150.
Ferguson SJ, Richardson DJ, Van Spanning RJM. Biochemistry and Molecular Biology of Nitrification. In: Biology of the Nitrogen Cycle (Bothe H, Ferguson SJ, Newton WE, eds.), (2007); Elsevier Science, Amsterdam, The Netherlands.
Tachiki T, Sakai K, Yamamoto K, Hatanaka M, Tochikura T. Conversion of nitrite to nitrate by nitrite-resistant yeasts. Agricultural and Biological Chemistry, (1988); 52(8): 1999-2005.
Zhang XY, Peng DC, Wan Q, Ju K, Wang BB, et al. Changing the nutrient source from ammonia to nitrate: Effects on heterotrophic bacterial growth in wastewater. Polish Journal of Environmental Studies, 2020; 29(2): 1473-1482.
Romano N, Zeng C. Acute toxicity of sodium nitrate, potassium nitrate, and potassium chloride and their effects on the hemolymph composition and gill structure of early juvenile blue swimmer crabs (Portunus pelagicus Linnaeus, 1758) (Decapoda, Brachyura, Portunidae). Environmental Toxicology and Chemistry, (2007); 26(9): 1955-62.
Prangnell DI, Samocha TM, Staresinic N. Water. In: Sustainable Biofloc Systems for Marine Shrimp (Samocha TM, ed.), (2019); 37. Academic Press, Elsevier Inc.
Burford MA, Williams KC. The fate of nitrogenous waste from shrimp feeding. Aquaculture, (2001); 198(1-2): 79-93.
Coelho RTI, Yasumaru FA, Passos MJACR, Gomes V, Lemos D. Energy budgets for juvenile Pacific white leg shrimp Litopenaeus vannamei fed different diets. Brazilian Journal of Oceanography, 2019; v67:e19243.
Lin YC, Chen JC. Acute toxicity of nitrite on Litopenaeus vannamei (Boone) juveniles at different salinity levels. Aquaculture, (2003); 224(1-4): 193-201.
Lin Y, Chen J. Acute toxicity of ammonia on Litopenaeus vannamei Boone juveniles at different salinity levels. Journal of Experimental Marine Biology and Ecology, (2001); 259(1): 109-119.
DOI: http://dx.doi.org/10.62940/als.v8i2.981
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