In-vitro Production of Cabbage and Cauliflower

Zahida Qamar, Ghulam Zahra Jahangir, Idrees Ahmad Nasir, Tayyab Husnain

Abstract


Background: An efficient method of artificial mass propagation was optimized for two very nutritious vegetables of Pakistan, the cabbage and cauliflower. Being an agrarian economy, Pakistan’s more than half of the population depends directly or indirectly on agricultural products.

Methodology: Hypocotyls of germinating of seeds (5-7 days old seedlings) were used as explants. Murashige and Skoog basal medium was supplemented with different concentrations of auxin (2,4 Dichlorophenoxy acetic  acid) and cytokinin (benzyl amino purine) in combination to study the callus forming tendency of cabbage and cauliflower; and found better if used in ratio of 2:1 (at least) respectively.

Results: For evaluation of regeneration potential Kinetin, Zeatin, Gibrellic acid-3, and Indole acetic acid were found good in combination with benzyl amino purine. Higher (than auxin) concentration of cytokinin was found essential to obtain good regeneration response of callus.

Conclusion:  Effectively concluded that these techniques can be used to raise the disease free stock of cabbage and cauliflower for genetic improvement of in-vitro bulk of varieties on commercial scale in a very brief time span.


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References


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DOI: http://dx.doi.org/10.62940/als.v1i2.32

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