Single Nucleotide Gene Polymorphism of Toll-Like Receptor 2 to Toxoplasmosis in Recurrent Aborted Women
Abstract
Background: This study was conducted at the College of Medicine – Al-Qadisiyah University in Cooperation with the women and children in Wasit hospitals from the period January to October -2022. It has been carried out on 102 blood samples from aborted women and congenital anomalies mothers referred to the hospitals 50 blood samples from pregnant women as control cases, and 50 blood samples as a control group obtained from healthy pregnant women. The objective was to determine the effect of TLR2 gene polymorphism on seropositive toxoplasmosis in aborted women
Methods: The research aimed to explore how the (TLR2) gene polymorphism influences the occurrence of miscarriages in women. This was investigated using the ARMS-PCR technique, which utilized allele primers to identify Toxoplasma gondii infection in women who experienced clinical miscarriages. The study also assessed the genotypes for the TLR2 G/A (rs1898830) SNPs through ARMS PCR assays, and all genotypes were evaluated for compliance with Hardy-Weinberg (H-W) equilibrium.
Results: All the analyzed genotypes preserved the H-W equilibrium among the studied groups of patients (P >0.942 and 0.893 ). The result of gene polymorphism of (TLR2, SNP rs-1898830) in Toxoplasma aborted women, showed that the homozygote mutant AA appeared 19, and heterozygote mutant GA appeared in 53was a risk factor for abortion in TLR2, and the difference was non-significant.
Conclusion: The current study concluded that genetic polymorphisms of TLR-2 had a risk factor role in toxoplasma patients, which many correlated with aborted women, rather than pregnant women.
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DOI: http://dx.doi.org/10.62940/als.v10i0.2173
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