Metal Sulfates and Microbial Food Treatment Alleviate the Oxidative Damage Caused by PEG-Induced Osmotic Stress in Cotton Plants

Semra Almas, Tijen Demiral Sert

Abstract


Background: Drought stress restricts cotton production, which causes a considerable loss of cotton yields worldwide. Plant activators improve crop production and ameliorate abiotic stresses. Thus, the effects of plant activators on the genotype of cotton (Gossypium hirsutum L.) Candia, which is resistant to drought, were investigated.

Methods: Plants were treated with the plant activators, and then osmotic stress was initiated by 30% PEG 6000 treatment for 13 days. Antioxidant enzyme activities such as superoxide dismutase (SOD), ascorbate peroxidase (APX), and peroxidase (POX) were measured along with growth, relative water content (RWC), osmotic potential, photosynthetic pigments, ion leakage, proline accumulation, malondialdehyde (MDA) content, and reactive oxygen species (ROS).

Results: Osmotic stress reduced shoot fresh weight (FW) and osmotic potential but increased root length of Candia. Activator treatment alleviated these effects. Photosynthetic pigment contents and cell membrane permeability were not affected by osmotic stress and activator treatments; proline accumulation and lipid peroxidation levels were enhanced by osmotic stress, but activators alleviated these increments. Activity bands for the isozymes Mn-SOD1, SOD2, and SOD3, as well as the isozyme Fe-SOD, were found in Candia. Osmotic stress elevated APX activity in Candia leaves, while activator treatment decreased it. POX activity was not affected by osmotic stress, but activator treatment decreased POX activity. Osmotic stress-induced accumulation of ROS was lessened by activator treatment.

Conclusion: Plant activator treatment reduced the amount of oxidative damage (ROS buildup and lipid peroxidation) caused by drought stress in the Candia genotype.

Keywords: Antioxidant system; Cotton; Gossypium hirsutum L.; Osmotic stress; PEG 6000; Plant activators  


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References


Pettigrew WT. Physiological Consequences of Moisture Deficit Stress in Cotton. Crop Science, (2004); 44:1265–1272.

Kim JH, Castroverde CDM, Huang S, Li C, Hilleary R. Increasing the Resilience of Plant Immunity to a Warming Climate. Nature, (2022); 607(7918): 339-344

Christmann A, Grill E, Huang J. Hydraulic Signals in Long-Distance Signaling. Current Opinion in Plant Biology, (2013); 16: 293–300.

Dumanovic´ J, Nepovimova E, Natic´ M, Kucˇa K and Jac´evic´ V. The Significance of Reactive Oxygen Species and Antioxidant Defense System in Plants: A Concise Overview. Frontiers in Plant Science, (2021); 11: 552969.

Turkan I, Demiral T. Recent Developments in Understanding Salinity Tolerance. Environmental and Experimental Botany, (2009); 67(1): 2-9.

Demiral T, Turkan I, Sekmen AH. Signaling Strategies during Drought and Salinity, Recent News. Advances in Botanical Research, (2011); 57: 293-317.

Zabalza A, Van Dongen JT, Froehlich A. Regulation of Respiration and Fermentation to Control the Plant Internal Oxygen Concentration. Plant Physiology, (2008); 149: 1087-1098.

Le Mouël C, Forslund A. How Can We Feed the World in 2050? Review of Responses from Global Scenario Studies. European Review of Agricultural Economics, (2017); 44(4):541-591.

Turkusay H, Tosun N, Yildiz S, Saygili H. Effects of Plant Activators on Physiological and Morphological Parameters of Processing Tomato. Acta Horticulturae, (2009); 808: 431-436.

Turkan I, Demiral T, Sekmen AH, Tosun N. Abiotic Stress and the Role of Plant Activators. In: Lyons TP, Jacques KA, editors: Proceedings of Alltech's 20th Annual Symposium on Nutritional Biotechnology in the Feed and Food Industries. (2004); 387-390. Nottingham University Press, England.

Yakhin OI, Lubyanov AA, Yakhin IA, Brown PH. Biostimulants in Plant Science: A Global Perspective. Frontiers in Plant Science, (2017); 7: 2049.

Calvo P, Nelson L, Kloepper JW. Agricultural Uses of Plant Biostimulants. Plant and Soil, (2014); 383: 3–41.

Sun T-J, Lu Y, Narusaka M, Shi C, Yang Y-B, Wu J-X, et al. A Novel Pyrimidin-Like Plant Activator Stimulates Plant Disease Resistance and Promotes Growth. PLoS One, (2015); 10(4): e0123227.

Dayan A, Sari N. The Effects of Different Plant Activators on Fruit and Seed Yield and Properties of Snack Pumpkin (Cucurbita pepo L.). International Journal of Scientific and Technological Research, (2019); 5(10): 1-12.

Smart RE, Bingham GE. Rapid Estimates of Relative Water Content. Plant Physiology, (1974); 53(2):258-260.

Lichtenthaler HK, Wellburn AR. Determination of Total Carotenoids and Chlorophylls A and B of Leaf Extracts in Different Solvents. Biochemical Society Transactions, (1983); 11: 591–592.

Bates LS, Waldren RP, Tear ID. Rapid Determination of Free Proline for Water-Stress Studies. Plant and Soil, (1973); 39:205-207.

Dionisio-Sese ML, Tobita S. Antioxidant Responses of Rice Seedlings to Salinity Stress. Plant Science, (1998); 135(1): 1-9.

Madhava-Rao KV, Sresty TVS. Antioxidative Parameters in the Seedlings of Pigeon Pea (Cajanus cajan L. Millspaugh) in Response to Zn and Ni Stresses. Plant Science, (2000); 157: 113-128.

Bradford MM. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry, (1976); 72: 248-254.

Laemmli UK. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature, (1970); 227(5259): 680-685.

Beauchamp C, Fridovich I. Superoxide Dismutase: Improved Assays and Applicable to Acrylamide Gels. Analytical Biochemistry, (1971); 44: 276-287.

Vitória AP, Lea PJ, Azevedo RA. Antioxidant Enzymes Responses to Cadmium in Radish Tissues. Phytochemistry, (2001); 57(5): 701-710.

Nakano Y, Asada K. Hydrogen Peroxide is Scavenged by Ascorbate-Specific Peroxidase in Spinach Chloroplasts. Plant and Cell Physiology (1981); 22(3): 867-880.

Herzog V, Fahimi HD. A New Sensitive Colorimetric Assay for Peroxidase Using 3, 3'-Diaminobenzidine as Hydrogen Donor. Analytical Biochemistry, (1973); 55: 554.

Bernt E, Bergmeyer HU. Inorganic Peroxidases. In: Bergmeyer HU, editor: Methods of Enzymatic Analysis. (1974); Vol. 4: 2246–2248. Academic Press, New York, USA.

Xu S, Li J, Zhang X, Wei H, Cui L. Effects of Heat Acclimation Pretreatment on Changes of Membrane Lipid Peroxidation, Antioxidant Metabolites, and Ultrastructure of Chloroplasts in Two Cool-Season Turfgrass Species under Heat Stress. Environmental and Experimental Botany, (2006); 56: 274–285.

Farooq M, Wahid A, Kobayashi NSMA, Fujita DBSMA, Basra SMA. Plant Drought Stress: Effects, Mechanisms, and Management: Sustainable Agriculture. (2009); 153-188. Springer, Dordrecht.

Wrona AF. Factors Affecting Water Use: Newsletter of the Cotton Physiology Education Program. (2000); Vol: 1 No: 1. National Cotton Council.

Ludlow MM, Muchow RC. A Critical Evaluation of Traits for Improving Crop Yields in Water-Limited Environments. Advances in Agronomy, (1990); 43: 107-153.

Shen J, Guo M, Wang Y, Yuan X, Wen Y, Song X, Dong S, Guo P. Humic Acid Improves the Physiological and Photosynthetic Characteristics of Millet Seedlings under Drought Stress. Plant Signaling and Behavior, (2020); 15(8): e1774212 (13 pages).

Michel BE, Kaufmann MR. The Osmotic Potential of Polyethylene Glycol 6000. Plant Physiology, (1973); 51 (5): 914–916.

Carter JE Jr, Patterson RP. Use of Relative Water Content as a Selection Tool for Drought Tolerance in Soybean: 1985 Agronomy Abstract. (1985); ASA. Madison, WI.

Nepomuceno A, Oosterhuis D, Stewart J. Physiological Responses of Cotton Leaves and Roots to Water Deficit Induced by Polyethylene Glycol. Environmental and Experimental Botany, (1998); 40(1), 29-41.

Mansour MMF. Nitrogen-Containing Compounds and Adaptation of Plants to Salinity Stress. Biologia Plantarum, (2000); 43: 491-500.

Farouk S, Al-Huqail AA. Sustainable Biochar and/or Melatonin Improve Salinity Tolerance in Borage Plants by Modulating Osmotic Adjustment, Antioxidants, and Ion Homeostasis. Plants, (2022); 11(6): 765.

Samaras Y, Bressan RA, Csonca LN, Garcia-Rios MG, Paino D, Urzo M, Rhodes D. Proline Accumulation During Drought and Salinity. In: Smirnoff N, editor: Environment and Plant Metabolism. (1995); 161-187. Bios Scientific Publishers, Oxford, UK.

Gomes FP, Oliva MA, Mielke MS, Almeida AAF, Aquino LA. Osmotic Adjustment, Proline Accumulation and Cell Membrane Stability in Leaves of Cocos nucifera Submitted to Drought Stress. Scientia Horticulturae, (2010); 126: 379-384.

Yildiztugay E., Ozfidan-Konakci C., Kucukoduk M. Exogenous Nitric Oxide (as Sodium Nitroprusside) Ameliorates Polyethylene Glycol-Induced Osmotic Stress in Hydroponically Grown Maize Roots. Journal of Plant Growth Regulation, (2014); 33: 683-696.

Baloğlu MC, Kavas M, Aydin G, Öktem HA, Yücel AM. Antioxidative and Physiological Responses of Two Sunflower (Helianthus Annuus) Cultivars under PEG-Mediated Drought Stress. Turkish Journal of Botany, (2012); 36: 707-714.

Gedeon S, Ioannou A, Balestrini R, Fotopoulos V, Antoniou C. Application of Biostimulants in Tomato Plants (Solanum lycopersicum) to Enhance Plant Growth and Salt Stress Tolerance. Plants, (2022); 11: 3082.

Wang Y, Branicky R, Noe A, Hekimi S. Superoxide Dismutases: Dual Roles in Controlling ROS Damage and Regulating ROS Signaling. Journal of Cell Biology, (2018); 217(6): 1915–1928.

Naboulsi I, Ben Mrid R, Ennoury A, Zouaoui Z, Nhiri M, Ben Bakrim W, Yasri A, Aboulmouhajir A. Crataegus oxyacantha Extract as a Biostimulant to Enhance Tolerance to Salinity in Tomato Plants. Plants, (2022); 11(10): 1283.

Abou-Sreea AIB, Azzam CR, Al-Taweel SK, Abdel-Aziz RM, Belal HEE, Rady MM, Abdel-Kader AAS, Majrashi A, Khaled KAM. Natural Biostimulant Attenuates Salinity Stress Effects in Chili Pepper by Remodeling Antioxidant, Ion, and Phytohormone Balances, and Augments Gene Expression. Plants (Basel), (2021); 10(11): 2316. PMID: 34834678; PMCID: PMC8617650.

Abdalla MM. Boosting the Growth of Rocket Plants in Response to the Application of Moringa oleifera Extracts as a Biostimulant. Life Sciences, 11: (2014); 1097-8135. http://www.lifesciencesite.com. 189.

Demiral T, Turkan I. Does Exogenous Glycinebetaine Affect Antioxidative System of Rice Seedlings under NaCl Treatment? Journal of Plant Physiology, (2004); 161: 1089–1100.




DOI: http://dx.doi.org/10.62940/als.v11i3.1739

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