Stressed Experimental diabetic Rats challenging with Glimepiride

Wissam Sajid Hashim, Youssef Shakuri Yasin, Emad Ayal Muter, Yousif Ahmed Khalil

Abstract


Background: This study was accomplished to evaluate the anticipated effects of glimepiride on some of the hematological parameters besides the antioxidant enzymes of the alloxan-induced diabetic and healthy rats.

Methods: In this study, thirty-two adult albino male rats were adopted. The animals were set in a random manner into four groups of eight rats each. The animals of the control group were dosed orally with 5ml distilled water. The second were injected intraperitoneally with 150 mg/kg of Alloxan one time to induce diabetes. The third group were administered a daily oral dose of 5 mg/kg of Glimepiride. The fourth group were injected with alloxan in the same manner as the second group and then dosed orally with 5mg/kg of glimepiride. The above-mentioned experimental protocol has extended for one month and thereafter the planned tests were done.

Results: The results showed that diabetes induced by alloxan led to significant decline in the packed cells volume (PCV), hemoglobin concentration (Hb), platelets (PLT), red blood cell counts (RBC), glutathione (GSH), alanine aminotransferase (ALT) and alkaline phosphatase (ALP)at (p≤0.05) with significant elevation in the total white blood cells count (WBC), malondialdehyde (MDA), erythrocytes sedimentation rate (ESR) and aspartate aminotransferase (AST) comparing with those of the control group. The use of glimepiride alone to the healthy rats led to significant decline in RBC,PLT, GSH and AST with significant elevation in ESR and WBC without an effect on the PCV, Hb, MDA, ALT and ALPat (p≤0.05) comparing with those of the control group. Treatment of alloxan-induced diabetic rats with glimepiride led to significant decline in RBC, HB and PCV with significant elevation in WBC, PLT, GSH, MDA and ALT at (p≤0.05) compared with the control group.

Conclusion: We conclude that glimepiride does affect the blood parameters and the antioxidant enzymes.

Keywords: Glimepiride; Amaryl; Stress; Diabetes; Rats    


Full Text:

PDF

References


Chikezie P, Ojiako O, Ogbuji A. Oxidative stress in diabetes mellitus. International Journal of Biological Chemistry, (2015); 9(3): 92-109.

Daya R, Bayat Z, Raal F.Effects of diabetes mellitus on health-related quality of life at a tertiary hospital in South Africa: A cross-sectional study.South African Medical Journal, (2016); 106(9): 918-928.

Kalra S. Place of sulfonylureas in the management of type 2 diabetes mellitus in South Asia. Indian Journal of Endocrinology and Metabolism, (2015); 19(5):577–596.

Nakamura I, Oyama J, Komoda H, Shiraki A, Sakamoto Y, Taguchi I, Hiwatashi A, Komatsu A, Takeuchi M, Yamagishi S, Inoue T, Node K. Possible effects of glimepiride beyond glycemic control in patients with type 2 diabetes: a preliminary report. Cardiovascular Diabetology, (2014); 13(15): 1-8.

Abou-seif M, Youssef A. Oxidative stress and male IGF-1, gonadotropin and related hormones in diabetic patients. Clinical Chemistry and Laboratory Medicine, (2001); 39 (7): 618-623.

Alu S, Los E, Ford A, Stone W. Oxidative Stress in Type 2 Diabetes: The Case for Future Pediatric Redoxomics Studies. Antioxidants, (2022); 11(7): 1-17.

Neto A, Silva I, Ivo M, Rodrigues C, Parisotto E, Ramalho R, Monteiro G.Effects of oxidative stress on liver, brain and spinal cord of rats using L-NAME and treated with hydroxyurea. A model of sickle cell complication. Acta Cirurgica Brasileira, (2020); 35(3): 1-6.

Alenzi F, Alshaya D. Biochemical and molecular analysis of the beta-globin gene on Saudi sickle cell anemia. Saudi Journal of Biological Sciences, (2019); 26(7):1377-84.

Salis A, Petrson R, Stecker M, Patal N, Willis L, Galley P, Eclavea A, Dreesen R. Suprarenal Intraarterial infusion of alloxan and streptozotocin during Balloon occlusion of the Juxtrarenal abdominal aorta: A simple technique for inducing Diabetes Mellitus in Canines with reduced mortality. Academic Radiology, (2001); 8: 473 – 477.

Siemianowicz K, Gminski J, Telega A, Wojcik A, Psielezna B, Bochenek R, Francus T. Blood antioxidant parameters in diabetic retinopathy. InternationalJournal of.Molecular Medicine,(2004); 14 (3): 433-437.

Frohnert B, Jacobs D, Steinberger J, Moran A, Steffen L, Sinaiko A. Relation between serum free fatty acids and adiposity, insulin resistance, and cardiovascular risk factors from adolescence to adulthood. Diabetes, (2013); 62: 3163–3169.

Yilidirim O. The effect of vitamin C and cobalt supplementation on antioxidant status in healthy and diabetic rats. African Journal of Biotechnology, (2009); 8(19): 5053-50.

Kromhauser C, Garcia R, Wrobel J. Serum SE and GPx concentration in type 2 diabetes mellitus patients. Primary Care Diabetes, (2008); 2(2): 81-85.




DOI: http://dx.doi.org/10.62940/als.v10i1.1595

Refbacks

  • There are currently no refbacks.