Toxicopathological effects of endosulfan in female Japanese Quails (Coturnix japonica)

Tahir Hussain, Muhammad Kashif Saleemi, Muhammad Zargham Khan, Ahrar Khan, Rao Zahid Abbas, Muhammad Qamar Bilal, Farrah Deeba, Hamid Irshad, Zahida Fatima, Farhan Afzal, Umar Farooq, Muhammad Moazam Jalees

Abstract


Background: The current study was planned to investigate the toxico-pathological effects of endosulfan in female Japanese quails.

Methods: A total of 120 quail of 4 weeks old were divided into six equal groups (A-F) and administered endosulfan in feed at dose rate of 0, 5, 25, 50, 100, and 500 mg/kg feed, respectively for 90 days. Parameters studied included clinical signs, feed intake, body weight and mortality. Hematology, serum biochemistry, hatchability and fertility were also determined. Gross and microscopic changes on different organs were recorded.

Results: The quails of the group B did not show any clinical signs and had significantly lower values of feed intake, testes relative weight and leukocyte number than those of the control group A. The quails of group C and D had mild depression while those of the group E and F showed nervous excitation following ingestion of endosulfan. There was a dose related delay in onset of crowing, appearance of foamy material in the droppings. The feed intake, erythrocyte and leukocyte counts, hematocrit values, and serum total proteins of endosulfan fed quails were significantly (p < 0.05) lower than that of the group A. The total egg production in groups A, B and C was significantly higher from group D, E and F.  The hatchability in group A and B was significantly higher from groups C, D, E and F. The difference of dead in shell % and early dead among different groups was nonsignificant. Infertile egg percentage was significantly higher in group E compared with all other groups except group F. The necrotic changes were observed in all parts of oviduct in high dose groups, similarly necrotic changes and vacuolar degeneration was observed in hepatic parenchyma in high dose groups D-F.

Conclusion: It may be concluded that endosulfan leads to dose dependent changes in the quails.

Keywords: Body weight; Coturnix japonica; Endosulfan; Haematological values; Histopathology


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References


Ahmad KR, Shehry K, Raees K et al., Adverse effects of cypermethrin on the chick (Galus domesticus) development are reversed by co-treatment with vitamin E and olive oil. Pakistan Veterinary Journal (2018); 38: 46-50.

AL-Ruwaili M, Alkhalaileh NI, Herzallah SM et al. Reduction of aflatoxin b1 residues in meat and organs of broiler chickens by lactic acid bacteria. Pakistan Veterinary Journal (2018); 38(3): 325-328.

Anand M, Agrawal AK, Gopal K et al. Endosulfan and cholinergic (muscarinic) transmission: effect on electroencephalograms and [3H] quinuclidinyl benzilate in pigeon brain. Environmental Research (1986); 40:421-6.

Ansari RA, MK Siddiqui, PK Gupta. Toxicity of endosulfan: distribution of alpha- and beta-isomers of racemic endosulfan following oral administration in rats. Toxicology Letters, (1984); 21(1):29-33.

Benjamin MM. Outline of veterinary clinical pathology (1978), 2nd Ed. The Iowa University press, USA.

Butt SL, Saleemi MK, Khan MZ et al. Cadmium toxicity in female Japanese quail (Coturnix japonica) and its diminution with silymarin. Pakistan Veterinary Journal, (2018); 38: 249-255.

Cable GG, Doherty S. Acute carbamate and organochlorine toxicity causing convulsions in an agricultural pilot: a case report. Aviat. Space Environmental Medicine, (1999); 70(1):68-72.

Chitra KC, Latchoumycandane C, Mathur PP. Chronic effects of endosulfan on the testicular functions of rat. Asian Journal of Andrology, (1999); 1:203-206.

Dalsenter PR, Dallegrave E, Mello JR, Langeloh A et al. Reproductive effects of endosulfan on male offspring of rats exposed during pregnancy and lactation. Human and Experimental Toxicology, (1999); 18(9):583-589.

Dikshith DS, Raizada RB, Kumar SN et al., Effect of repeated dermal application of endosulfan to rats. Veterinary and Human Toxicology, (1988); 30(3):219-224.

Gill TS, Pande J, Tewari H. Effects of endosulfan on the blood and organ chemistry of freshwater fish, Barbus conchonius Hamilton. Ecotoxicology and Environmental Safety, (1991); 21(1):80-91.

Gul ST, Khan A, Farooq M, Niaz S et al., Effect of Sub Lethal Doses of Thiamethoxam (A Pesticide) on Hemato-Biochemical Values in Cockerels. Pakistan Veterinary Journal, (2017); 37(2): 135-138.

Gul ST, Khan A , Ahmad M, Anwar MF et al., Effect of sub-lethal doses of thiamethoxam (a neonicotinoid) on hemato-biochemical parameters in broiler chicks. Toxin Reviews, (2018); 37(2): 144–148.

Hill, EF, Camardese MB. Lethal Dietary Toxicities of Environmental Contaminants to Coturnix, Technical Report Number 2. U.S. Department of Interior, Fish and Wildlife Service, Washington, (1986); DC. 6-55.

Hussain R, Ali F, Rafique A, Ghaffar A et al., Exposure to sub-acute concentrations of glyphosate induce clinico-hematological, serum biochemical and genotoxic damage in adult cockerels. Pakistan Veterinary Journal, (2019); 39(2): 181-186.

Hussain R, Ghaffar A, Ali HM, Abbas RZ et al., Analysis of different toxic impacts of Fipronil on growth, hemato-biochemistry, protoplasm and reproduction in adult cockerels, Toxin Reviews, (2018); 37:4, 294-303

Johnson WW, Finley MT. Handbook of Acute Toxicity of Chemicals to Fish and Aquatic Invertebrates, Resource Publication 137. U.S. Department of Interior, Fish and Wildlife Service, Washington, (1980); DC, 6-56.

Lo RS, Chan JC, Cockram CS, Lai FM. Acute tubular necrosis following endosulfan insecticide poisoning. Journal of Toxicology Clinical Toxicology, (1995); 33(1):67-9.

Michael Fry D. Reproductive Effects in Birds Exposed to Pesticides and Industrial Chemicals. Environmental Health Perspectives, (1995); 103(7).

Naqvi SM Vaishnavi C. Bioaccumulative potential and toxicity of endosulfan insecticide to non-target animals. Comparative Biochemistry and Physiology, (1993); 105(3):347-361.

Naseem MN, Saleemi MK, Abbas RZ et al., Hematological and serum biochemical effects of aflatoxin B1 intoxication in broilers experimentally infected with fowl adenovirus-4 (FAdV-4). Pakistan Veterinary Journal,(2018); 38(2): 209-213

Qamar AB, Khan MZ, Khan A, Saleemi MK, Javed I. Endosulfan (Thiodan@ EC 35) induced toxicopathological effects in male Japanese quails (Coturnix japonica). Journal of National Science Foundation of Sri Lanka, (2012); 40: 97-105.

Pandey AC. Impact of endosulfan (thiodan) EC 35 on behavior and dynamics of oocyte development in the teleostean fish, Colisa (Trichogaster) fasciatus. Ecotoxicology and. Environmental Safety, (1988); 15:221-225.

Papov. Embryo and genotoxic effects of two endosulfan forms in the culture of rat and mouse pre- and postimplantation embryos. Ontogenez, (1998); 29: 104-112.

Riaz A, Ulhaq M, Khan IA, Khan A et al., Chlorpyrifos induced dermal toxicity in albino rabbits. Pakistan Veterinary Journal, (2018) 38(1): 91-95.

Shalini-Singh, PD, Kumar S. Biodegradation of alpha and beta isomers of endosulfan and endosulfan sulphate in Indian soils. Journal of Environmental Sciences and Health, (2000); 5(3):337-346.

Singh, SK and Pandey RS. Toxicity of endosulfan on kidney of male rats in relation to drug metabolizing enzymes and microsomal lipid peroxidation. Indian Journal of Experimental Biology, (1989); 27(8):725-8.

Smith, AG. Chlorinated Hydrocarbon Insecticides. In Handbook of Pesticide Toxicology. Hayes, WJ, Jr and Laws, ER, Jr, Eds. Academic Press Inc., New York, (1991); 6-3.

Subhani Z, Shahid M, Hussain F and Khan JA, Efficacy of Chlorella pyrenoidosa to ameliorate the hepatotoxic effects of Aflatoxin B1 in broiler chickens. Pakistan Veterinary Journal, (2018) 38: 13-18

Venkateswarlu, K, Suryarao K, Srinivas V et al., Endosulfan poisoning–a clinical profile. J Assoc. Physicians India, (2000); 8(3):323-5.




DOI: http://dx.doi.org/10.62940/als.v7i2.866

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