Investigation of the Impact of Environmentally Friendly prepared Alumina Nanoparticles on Bacterial Activity

Taqwa Gh. Hamad, Taghried Ali Salman

Abstract


Background: Staphylococcus aureus, which is known as the most significant nosocomial pathogen and frequently causes postoperative wound infections, is a serious health issue in hospitals. Staphylococcus aureus has become increasingly drug resistant, and most of its strains have been shown to be resistant to practically all antibiotics. Numerous medical applications of aluminum oxide have been investigated, and this research indicates that the growth of Staphylococcus aureus may be inhibited in the presence of aluminum oxide nanoparticles.

Methods: The study was conducted from March 2023 to the end of July 2023 at Al-Aziziya Hospital in Iraq. Using spectrophotometry on microtiter plates, biofilms were created in vitro. Single isolated organisms of Staphylococcus aureus were examined for antibiotic susceptibility patterns using a conventional disk diffusion method. Furthermore, Prepared aluminum oxide nanoparticles were characterized using various techniques.

Results: The results show that strongly inhibited growth of Staphylococcus aureus in the presence of aluminum oxide nanoparticles after 24 hours at 0.06125,0.1225 0.245, and 0.49 M. On the other hand, X-ray diffraction analysis revealed an average crystallite size of 35 nm for the aluminum oxide nanoparticles. The FT-IR spectrum displayed prominent peaks at 615 and 636, corresponding to the stretching vibrations of aluminum oxide. The EDX measurements confirmed the presence of aluminum (Al) and oxygen (O) peaks, indicating the purity of the sample.

Conclusions: The antimicrobial assay demonstrated that the aluminum oxide nanoparticles exhibited significant antibacterial activity against Staphylococcus aureus. At a concentration of 0.06125 M of Al2O3, Staphylococcus aureus displayed a maximum zone of inhibition measuring 39 mm.


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References


Arakelyan S, Veiko V, Kutrovskaya S, Kucherik A, Osipov A, Vartanyan T, Itina T. Reliable and well-controlled synthesis of noble metal nanoparticles by continuous wave laser ablation in different liquids for deposition of thin films with variable optical properties. Journal of Nanoparticle Research, (2016);18:1-12.

Baalousha M, Fshinnia A, Guo L. Natural organic matter composition determines the molecular nature of silver nanomaterial-NOM corona. Environmental Science: Nano, (2018); 5(4): 868-881.

Neethu S, Midhun S, Radhakrishnan E, Jyothi’s M. Green synthesized silver nanoparticles by marine endophytic fungus Penicillium polonium and its antibacterial efficacy against biofilm forming, multidrug-resistant Acinetobacter Bahmani. Microbial pathogenesis, (2018); 116: 263-272.

Devi L, Joshi S. Ultrastructure of silver nanoparticles biosynthesized using endophytic fungi. Journal of Microscopy and Ultrastructure, (2015); 3(1): 29-37.

Baghayeri M. Green synthesis of silver nanoparticles using water extract of Salvia leriifolia: Antibacterial studies and applications as catalysts in the electrochemical detection of nitrite. Applied Organometallic Chemistry, (2017).

Bindhu M, Umadevi M. Antibacterial and catalytic activities of green synthesized silver nanoparticles. Spectrochemical Acta Part A: Molecular and Biomolecular Spectroscopy, (2015);135: 373-378,

Edison T, Lee Y, Sethuraman M. Green synthesis of silver nanoparticles using Terminalia cuneata and its catalytic action in reduction of direct yellow-12 dye. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, (2016);161: 122-129.

Ibrahim T, Taghried A, Salma A. Effectiveness of Magnesium oxide Nanoparticles in the Management of Thyroid Hormone Level. Egyptian Journal of Chemistry, (2021);64 (6): 2889 -2894.

Gudkov S, Burmistrov D, Smirnova V, Semenova A, Lisitsyn A. A Mini Review of Antibacterial Properties of Al2O3 Nanoparticles. Nanomaterials, (2022);12(15): 2635-2641.

Singh S, Gill A, Nlooto M, Karpoormath R. Prostate cancer biomarkers detection using nanoparticles based electrochemical biosensors. Biosensors and Bioelectronics, (2019);137, 213-221.

Vimala D, Murugesan R, Francesco M, Antara B, Xiao F, Surajit P. Comparative study on anti-proliferative potentials of zinc oxide and aluminum oxide nanoparticles in colon cancer cells. Acta BioMedica, (2019); 90(2): 241.

Spirescu V, Chircov C, Grumezescu A, Vasile B, Andronescu E. Inorganic nanoparticles and composite films for antimicrobial therapies. International Journal of Molecular Sciences, (2021); 22(9): 459-464.

Xu, C. Applications of iron oxide-based magnetic nanoparticles in the diagnosis and treatment of bacterial infections. Frontiers in Bioengineering and Biotechnology, (2019); 7: 1–15.

Parks M, Messmer T. Characteristics of electronic cigarette users and their smoking cessation outcomes. Cancer, (2015);121: 800-810.

Raghunath A, Perumal E. Metal oxide nanoparticles as antimicrobial agents: a promise for the future. International journal of antimicrobial agents, (2017); 49(2): 137-152.

Marwah H, Taghried A. Kinetic and inhibition effect studies of ecofriendly synthesized silver nanoparticles on lactate dehydrogenase and ferritin activity of waxy crude oil. Egyptian Journal of Chemistry, (2022); 65(6): 627 – 635.

Bobkov Y, Walker W, Cattaneo A. Altered functional properties of the codling moth Orco mutagenized in the intracellular loop 3. Scientific Reports, (2021); 11(1):1-16.

Gudkov S, Burmistrov D, Serov D, Rebezov M, Semenova A, Lisitsyn A. Do iron oxide nanoparticles have significant antibacterial properties. Antibiotics, )2021(;10(7): 884-889.

Akhtar S, Shahzad K, Mushtaq S, Ali I, Rafe M, Fazal-ul-Karim S. Antibacterial and antiviral potential of colloidal Titanium dioxide (TiO2) nanoparticles suitable for biological applications. Materials Research Express, (2019); 6(10): 105409.

Shah A, Haq S, Rehman W, Waseem M, Shoukat S, Rehman M. Photocatalytic and antibacterial activities of Paeonia emodin mediated silver oxide nanoparticles. Materials Research Express, (2019); 6(4): 04200.

Mohamed A. Eco-friendly Myogenic Synthesis of ZnO and CuO Nanoparticles for in vitro antibacterial, antibiofilm, and antifungal applications. Biological Trace Element Research, (2021); 199(7): 2788-2799.

Sara H, Taghreid A. The influence of green synthesized zinc oxide nanoparticles on testosterone hormone. Biochemical and Cellular Archives, (2022); 22(1): 921-928.

Sliwinska A, Kwiatkowski D, Czarny P, Milczarek J,Toma M, Korycinska A, Szemraj J, Sliwinski T. Genotoxicity and cytotoxicity of ZnO and Al2O3 nanoparticles. oxicology Mechanisms and Methods, (2015); 25: 176–183.

Jiao W, Yue M, Wang Y. Synthesis of morphology-controlled mesoporous transition alumina derived from the decomposition of alumina hydrates. Microporous and Mesoporous Materials, (2012); 147:167–177.

Manikandan V, Jayanthi P, Priyadharsan A, Vijayapratha E, Anbarasan P, Velmurugan P. Green synthesis of pH-responsive Al2O3 nanoparticles: Application to rapid removal of nitrate ions with enhanced antibacterial activity. Journal of Photochemistry and Photobiology A: Chemistry, (2019); 371:205–215.

Nasrollahzadeh M, Issaabadi Z, Sajadi M. Green synthesis of Cu/Al2O3 nanoparticles as efficient and recyclable catalyst for reduction of 2,4-dinitrophenylhydrazine, Methylene blue and Congo red. Composites Part B: Engineering, (2019); 166:112–119.

Ezati F, Sepehr E, Ahmadi F. The efficiency of nano-TiO2 and γ-Al2O3 in copper removal from aqueous solution by characterization and adsorption study. Scientific Reports, (2021); 11:18831.

Saliani M, Honarbakhsh A, Zhiani R, Movahedifar S, Motavalizadehkakhky,A. Effects of GO/Al2O3 and Al2O3 nanoparticles on concrete durability against high temperature, freeze-thaw cycles, and acidic environments. Advances in Civil Engineering, (2021); 1-12.

Kalneus V, Nemushchenko D, Larichkin V, Briutov A. Research of Physical and Mechanical Properties of Fly Ash Ceramics with SiO2 and Al2O3 Nanoparticles as Functional Addition. In Key Engineering Materials, (2021); 887: 528-535.

Sara H, Taghried A. The potential of green-synthesized copper oxide nanoparticles from coffee aqueous extract to inhibit testosterone hormones. Egyptian Journal of Chemistry, (2022); 65(4): 395 – 402.

Ahmed S, Ahmad M, Swami B, Ikram S. Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. Journal of radiation research and applied sciences, (2016); 9(1): 1-7.

Numan A, Ahmed M, Galil M, Al-Qubati M, Raweh A, Helmi E. Bio-Fabrication of silver nanoparticles using Catha edulis extract: procedure optimization and antimicrobial efficacy encountering antibiotic-resistant pathogens. Advances in Nanoparticles, (2020); 11(2): 31-54.

Bindhu M, Umadevi M, Esmail G, Al-Dhab N, Arasu M. Green synthesis and characterization of silver nanoparticles from Moringa oleifera flower and assessment of antimicrobial and sensing properties. Journal of Photochemistry and Photobiology B: Biology, (2020); 205: 111836.




DOI: http://dx.doi.org/10.62940/als.v10i0.2111

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