Comparison between some genera of Grammosciadium and Torilis belonging to the (Apiaceae) family using nucleotide sequence of Matk gene into a protein

Nagham Thayer Salman, Ghassan Radam Idan Al.Taie, Ali Mala Khedir Galalaey

Abstract


Background: The amino acid composition of certain species of the Grammosciadium and Torilis genera, belonging to the Apiaceae or Umbelliferae family, is investigated. This family of flowering plants is named after the genus Apium and is commonly referred to as the celery or carrot family. These plants are found in the wild in temperate regions and the northern regions of Iraq.

Methods: The research was carried out in the Molecular Genetics Laboratory at the College of Education for Pure Sciences, University of Diyala, Iraq. A total of 40 plant samples were collected from various places in Iraq, with 10 samples obtained for each species. The pliable specimens were enclosed in nylon pouches, stored in the refrigerator, and conveyed to the laboratory for the explicit objective of DNA extraction, with the subsequent data being duly documented. The molecular systematic research on Apioideae has involved international collaboration. The ongoing investigations will focus on analyzing the remaining 200 or so genera that have not been reviewed previously, particularly those that have been difficult to classify phylogenetically. Additionally, the current tribes and clades will be re-examined and improved. The current and upcoming updates to all of these well-known groups, which will include both molecular and morphological information, will finally lead to the creation of a new classification for the subfamily.

Results: The results demonstrated a distinction in nucleotide sequences of the Matk gene across two genera as well as between species belonging to the same genus. Specifically, there were variations in the arrangement of 20 amino acids. The pH value of 14.03 was recorded as the maximum for citric acid in G. daucoides.

Conclusion: The commonality lies in the relative quantities of certain amino acids. Members of the same genus, as well as people belonging to the same species.

Keywords: Grammosciadium daucoides; Grammosciadium platycarpum; Torilis arvensis; Torilis chrysocarpa; Apiaceae (Umbelliferae); Amino acids; Molecular (gene Matk) 


Full Text:

PDF

References


Redouan F, Benítez G, Picone R, Crisafulli A, Yebouk C, Bouhbal M, Merzouki A. Traditional medicinal knowledge of apiaceae at talassemtane national park (northern Morocco). South African Journal of Botany, (2020);131(3):118-130. ‏

Al-Joboury K. Checklist of the Umbelliferae family in the herbarium of Iraq natural history museum. GSC Biological and Pharmaceutical Sciences, (2021); 15(3):177-181. ‏

Amiri M, Joharchi M. Ethnobotanical knowledge of Apiaceae family in Iran: A review. Avicenna journal of phytomedicine, (2016); 6(6):621. ‏

BACZYŃSKI J, MIŁOBĘDZKA A, BANASIAK Ł. Morphology of pollen in Apiales (asterids, eudicots). Phytotaxa, (2021); 478(1):1-32. ‏

Bittrich V, Kadereit J. Introduction to the orders of this volume, The Families and Genera of Vascular Plants,(2018);15(2):1-8. ‏

Calviño C, Downie S. Circumscription and phylogeny of Apiaceae subfamily Saniculoideae based on chloroplast DNA sequences. Molecular Phylogenetics and Evolution, (2007); 44(1):175-191. ‏

Nicolas A, Plunkett G. The demise of subfamily Hydrocotyloideae (Apiaceae) and the re-alignment of its genera across the entire order Apiales. Molecular Phylogenetics and Evolution, (2009); 53(1):134-151. ‏

Downie S, Spalik K, Katz-Downie D, Reduron J. Major clades within Apiaceae subfamily Apioideae as inferred by phylogenetic analysis of nrDNA ITS sequences. Plant Diversity and Evolution, (2010); 128(1):111. ‏

Banasiak Ł, Piwczyński M, Uliński T, Downie S, Watson M, Shakya B, Spalik K. Dispersal patterns in space and time: a case study of Apiaceae subfamily Apioideae. Journal of Biogeography, (2013); 40(7):1324-1335. ‏

Ghazanfar S, McDaniel T. Floras of the Middle East: A quantitative analysis and biogeography of the flora of Iraq. Edinburgh Journal of Botany, (2016); 73(1):1-24. ‏

Aćimović, MG. Nutraceutical Potential of Apiaceae,Reference Series in Phytochemistry, (2019);12(3): 1311–1341.

Aćimović MG, Milić NB. Perspectives of the Apiaceae Hepatoprotective Effects – A Review. Nat Prod Commun. (2017);12(2):309-317.

Harborne J. Methods of plant analysis. In: Phytochemical methods: a guide to modern techniques of plant analysis, springer science & business media, (1984);3(2):1-36. ‏

Calvente Morais, Maristela, Torres, Luana, Kuramae, Eiko, Andrade, Sara, Mazzafera, Paulo. Plant grafting: Maximizing beneficial microbe-plant interactions, Rhizosphere, (2023); 29. 100825.

Carey F, Sundberg R. Chemical bonding and structure. Advanced Organic Chemistry: Part A: Structure and Mechanisms, Springer Science & Business Medi, (2000);1-73. ‏

Lewis T, Stone WL. Biochemistry, Proteins Enzymes. Treasure Island (FLExamining the utility of DNA barcodes for the identification of tallgrass prairie flora. Applications in Plant Sciences, (2021); 9(1): e11405.

‏18. Havill N, Foottit R, von Dohlen C. Evolution of host specialization in the Adelgidae (Insecta: Hemiptera) inferred from molecular phylogenetics. Molecular phylogenetics and evolution, (2007); 44(1):357-370. ‏

Abdel Nawab F. Molecular biology, academic library, first Arabic edition, Cairo, (1993); 97.

Shakara MD. Genetics, Amman, Dar Al Masirah for Publishing and Distribution, first edition, Amman – Jordan, (1999); 261-264.

Pimenov MG, Leonov MV. The genera of the Umbelliferae: a nomenclator. Kew: Royal Botanic Gardens, Kew Downie SR., Katz Downie DS., Spalik K. A phylogeny of Apiaceae tribe Scandiceae: evidence from nuclear ribosomal DNA internal transcribed spacer sequences, American Journal of Botany, (2000); 87(2): 76–95.

Downie SR, Katz DS, Watson MF. A phylogeny of the flowering plant family Apiaceae based on chloroplast DNA rpl16 and rpoC1 intron sequences: towards a suprageneric classification of subfamily Apioideae, American Journal of Botany, (2000); 87(3): 273–292.

Spalik K . Wojewódzka A. Downie S. The evolution of fruit in Scandiceae subtribe Scandicinae , Canadian Journal of Botany, (2001); 79(11): 1358-1374.




DOI: http://dx.doi.org/10.62940/als.v11i1.1972

Refbacks

  • There are currently no refbacks.