Phytochemical Screening of leaves of Polyalthia sclerophylla using Classical Methods and GC-Mass Spectroscopy: Cytotoxicity and Antibacterial Activities

Zahraa Sabbar Omran, Hend Ahmed Abbas, Farqad A. Albaidhani, Farqad A. Albaidhani, Huda M. Almusawi, Mustafa Mudhafar, Mustafa M. Karhib, Hasan Ali Alsailawi

Abstract


Background: Polyalthia sclerophylla (P. sclerophylla) is a member of the Annonaceae family, with a wide distribution in tropical and subtropical regions. In traditional Chinese medicine, various members of this genus have been employed as medicinal plants to address refractory ailments. The current study aims to extract the leaves of Polyalthia sclerophylla (TLPS) and determine their chemical contents using GS-Mass and standard phytochemical techniques.

Methods: The antibacterial and cytotoxicity activities of TLPS were used to evaluate its bio-medical characteristics. In that sequence, three solvents were used to extract TLPS to produce three samples: MTLPS, DTLPS, and HTLPS.

Results:  Phytochemical analysis indicated terpenoids and glycosides in all prepared models, but no alkaloids were observed. The GC-Mass data showed twenty-one chemical compounds. Pyridine, 2-Undecanol, 2-methyl-2-oxiranyl- and cyclobutanone were observed with higher percentages, while the 2(5H)-Furanone, 5-methyl, 1,1,3-Trimethylcyclopentane and 1,6-Heptadiene were observed with lower rates. The cytotoxicity study of TLPS was performed using Alamar blue assay using MG-63 cells. However, results show no detrimental influence at any dosage as cell availability increased. MTLPS, DTLPS and HTLPS were treated with six bacteria pathogens, and all of them showed significant effects against these bacteria to inhibit the growth of the bacteria. The extract of methanol (MLPS) was more effective in inhibiting the development of the bacteria compared with the DTLPS and HTLPS.

Conclusion: The current work has shown that the chemical composition of crude TPLS varies, leading to notable chemical, biological, and medicinal features, and their non-toxic impact.

Keywords: Polyalthia sclerophylla; GS-Mass; Antibacterial; Cytotoxicity activity 


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Firdous SM, Ahmed SN, Hossain SM, Ganguli S, Fayed MA. Polyalthia longifolia: phytochemistry, ethnomedicinal importance, nutritive value, and pharmacological activities review. Medicinal Chemistry Research, (2022);31(8):1252-64.

Dashora A, Rathore K, Raj S, Sharma K. Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen. Biochemistry and Biophysics Reports, (2022); 31(9):101320.

Kolaprath MK, Benny L, Varghese A. A facile, green synthesis of carbon quantum dots from Polyalthia longifolia and its application for the selective detection of cadmium. Dyes and Pigments, (2023); 210(2):111048.

Annapureddy PK, Kishore N. Non-isothermal pyrolysis of Polyalthia longifolia using thermogravimetric analyzer: Kinetics and thermodynamics. Journal of Renewable and Sustainable Energy, (2023); 15(5):053101.

Ghous M, Dogar Na, Hanif A, Jabbar M. Phytochemical Analysis And Anti-Oxidant Potential Of Ethanolic Extract Of Polyalthia Longifolia Leaves. Pakistan Journal of Science, (2023);75(02):434-8.

Zhangxin Y, Xinming S, Yuan Y, Jun Y, Shuo Y, Xiaobao L. Anti-inflammatory Clerodane Diterpenoids from Polyalthia longifolia. Chinese Journal of Organic Chemistry, (2023);43(2):751.

Siddappa RY, Aditya Rao SJ, Usha BM, Verma B, Mahadevappa P. Anti-proliferative Activity of Labdane Diterpenes Isolated from Polyalthia cerasoides and their Molecular Interaction Studies. Current Drug Discovery Technologies, (2022);19(5):78-85.

Zareen S, Adnan M, Khan SN, Alotaibi A. Anti-plasmodial potential of selected medicinal plants and a compound Atropine isolated from Eucalyptus obliqua. Open Chemistry, (2023);21(1):20220281.

Maulana I, Fasya D, Ginting B. Biosynthesis of Cu nanoparticles using Polyalthia longifolia roots extracts for antibacterial, antioxidant and cytotoxicity applications. Materials Technology, 2022;37(13):2517-21.

Lo IW, Liao GY, Lee JC, Chang CI, Wu YC, Chen YY, Liu SP, Su HJ, Liu CI, Kuo CY, Lin ZY. Novel Aporphine-and Proaporphine–Clerodane Hybrids Identified from the Barks of Taiwanese Polyalthia longifolia (Sonn.) Thwaites var. pendula with Strong Anti-DENV2 Activity. Pharmaceuticals, (2022);15(10):1218.

Mudhafar M, Zainol I. Medical values, antimicrobial, and anti fungal activities of Polyalthia genus. International Journal of Pharmaceutical Research, (2019) ;11(1): 90–96

Firdous SM, Ahmed SN, Hossain SM, Ganguli S, Fayed MA. Polyalthia longifolia: phytochemistry, ethnomedicinal importance, nutritive value, and pharmacological activities review. Medicinal Chemistry Research, (2022); 31(8):1252-64.

Shinde PK, Kokate RH, Gawade GS. Physicochemical, phytochemical, biological and chromatographic evaluation of Polyalthia longifolia plant leaves-A review. Research Journal of Science and Technology, (2023);15(1):41-8.

Alsailawi HA, Mudhafar M, Hanan AH, Ayat SS, Dhahi SJ, Ruaa KM, Raheem HA. Phytochemical screening and antibacterial activities of antiaris toxicaria stem, Polyalthia rumphii leaves and Polyalthia bullata stem extracts. InAIP Conference Proceedings, (2023); 2845 (1). AIP Publishing.

Ghous M, Dogar NA, Hanif A, Jabbar M. PHYTOCHEMICAL ANALYSIS AND ANTI-OXIDANT POTENTIAL OF ETHANOLIC EXTRACT OF POLYALTHIA LONGIFOLIA LEAVES. Pakistan Journal of Science, (2023);75(02):434-8.

Bhatt B, Chaurasia H, Singh R, Kaushik S. Phytochemical Profile and in vitro Sun-Protective Activity of Polyalthia longifolia (Sonn.) Thwaites Bark Extracts. Trop J Nat Prod Res, (2022);6(8):1174-7.

Dashora A, Rathore K, Raj S, Sharma K. Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen. Biochemistry and Biophysics Reports, (2023);1:101320.

Mudhafar M, Alsailawi HA, Zorah M, Karhib MM, Zainol I, Kadhim FK. Biogenic Synthesis and Characterization of AgNPs Using CEPS: Cytotoxicity and Antibacterial Activites. Journal of Advanced Research in Fluid Mechanics and Thermal Sciences, (2023);106(1):65-75.

Baqir S. Exploration of Antimicrobial Activities of an Ethnobotanically important Tree Terminalia arjuna of Family Combretaceae. GU Journal of Phytosciences, (2022);2(4):228-34.

Parusnath M, Naidoo Y, Singh M, Rihan H, Dewir YH. Phytochemical Composition of Combretum molle (R. Br. ex G. Don.) Engl. & Diels Leaf and Stem Extracts. Plants, (2022);12(8):1702.

Iyekowa O, Uwumarongie OH, Emmanuel KC, Omoagbotse PI, Okhions SO, Izuegbunam CL, Oligie MH, Eduwuirofo LO, Iyekekpolor RM, Okafor VC, Michael OT. Phytoconstituents, Acute Toxicity and In-Vivo Anti-anxiety Activity of Chloroform and Ethylacetate Extracts of Datura Stramonium in Balb/C Mice. Journal of Science and Technology Research, (2023);5(1):158-70.

Shree TJ, Poompavai S, Begum SM, Gowrisree V, Hemalatha S. Cancer-fighting phytochemicals: another look. J Nanomedine Biotherapeutic Discov. (2019);8:162:1-7.

Mudhafar M, Zainol I, Alsailawic HA, Karhib MM, Zorah M, Alnagdi FH, Hamzah MS. Bioactive chemical constituents of three crude extracts of Polyalthia Sclerophylla using GC-mass and phytochemical screening and their antibacterial and cytotoxicity activities. Eurasian Chemical Communications, (2023);5:675-90.

Shaikh JR, Patil MK. Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies, (2020);8(2):603-8.

Siddappa RY, Aditya Rao SJ, Usha BM, Verma B, Mahadevappa P. Anti-proliferative Activity of Labdane Diterpenes Isolated from Polyalthia cerasoides and their Molecular Interaction Studies. Current Drug Discovery Technologies, (2022);19(5):78-85.

Ahmed M, Ji M, Qin P, Gu Z, Liu Y, Sikandar A, Iqbal MF, Javeed A. Phytochemical screening, total phenolic and flavonoids contents and antioxidant activities of Citrullus colocynthis L. and Cannabis sativa L. Appl. Ecol. Environ. Res, (2019);17(3):6961-79.

Hossain MA, AL-Raqmi KA, Al-Mijizy ZH, Weli AM, Al-Riyami Q. Study of total phenol, flavonoids contents and phytochemical screening of various leaves crude extracts of locally grown Thymus vulgaris. Asian Pacific journal of tropical biomedicine, (2013);3(9):705-10.

Kujur RS, Singh V, Ram M, Yadava HN, Singh KK, Kumari S, Roy BK. Antidiabetic activity and phytochemical screening of crude extract of Stevia rebaudiana in alloxan-induced diabetic rats. Pharmacognosy research, (2010);2(4):258.

Kaur H, Amini MH, Prabhakar PK, Singh A, Suttee A. Phytochemical screening and antimicrobial activity of Caesalpinia sappan L. leaves. International Journal of Pharmacognosy and Phytochemical Research, (2016);8(6):1040-5.

Maskam NA, NH MR, HH MH. Phytochemicals screening and antioxidant activity of three different solvent extracts of Euodia redleyi leaves. Mod. Agric. Sci. Technol, (2017);3(5):18-21.

Arzumand Ara M, Saleh-e-In M, Ahmed NU, Ahmed M, Abul M. Phytochemical Screening, Analgesic, Antimicrobial and Anti-oxidant Activities of Bark Extracts of Adenanthera pavonina L.(Fabaceae). Advances in Natural and Applied Sciences, (2010);4(3):352-60.

Mudhafar M, Zainol I, Alsailawi HA, Zorah M, Karhib MM. Preparation and characterization of FsHA/FsCol beads: Cell attachment and cytotoxicity studies, Heliyon. (2023);9(5).

Dhawan D, Gupta J. Research article comparison of different solvents for phytochemical extraction potential from datura metel plant leaves. International Journal of Biological Chemistry 2017);11(1):17-22.

Hemagirri M, Sasidharan S. In vitro antiaging activity of polyphenol rich Polyalthia longifolia (Annonaceae) leaf extract in Saccharomyces cerevisiae BY611 yeast cells. Journal of ethnopharmacology, 2022;290):115110.

Zorah M, Mudhafar M, Majhool AA, Abbood SF, Alsailawi HA, Karhib MM, Mustapa IR. Review of the Green Composite: Importance of Biopolymers, Uses and Challenges. Journal of Advanced Research in Fluid Mechanics and Thermal Sciences, (2023);111(1):194-216.

Vinay, K. Antibacterial activity of crude extracts of Spirulina platensis and its structural elucidation of bioactive compound. Journal of Medicinal Plants Research, (2011);5(32), 7043-7048.

Alja’afreh IY, Alaatabi RM, Hussain Aldoghachi FE, Mudhafar M, Almashhadani HA, Kadhim MM, Hassan Shari F. Study the antioxidant of Matricaria chamomilla (Chamomile) powder: In vitro and vivo. Revis Bionatura, (2023); 8 (1) 63.




DOI: http://dx.doi.org/10.62940/als.v11i1.1740

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