Green Nano-synthesis: Salix alba Bark-Derived Zinc Oxide Nanoparticle and their nematicidal Efficacy against root knot nematode Meloidogyne incognita

Sidra Ahmad, Mian Afaq Ahmad, Fazal Umar, Iqbal Munir, Neelum Iqbal, Nayab Ahmad, Gulzar Ahmad, Muhammad Ijaz, Zia ur Rahman, Syed Jehangir Shah, Hamza Iqbal, Ateeq Ur Rehman

Abstract


Background: Plant parasitic nematodes (PPNs) are considered major agriculture pests, causing significant damage to crops by directly targeting the plant root system to prevent water and nutrient uptake. One of its major species, Meloidogyne incognita, is considered a serious threat to agriculture crop production worldwide. The current study was intended to evaluate the potential of Nanoparticles synthesized from Salix alba bark extract as nematicidal agent.

Methods: Phytochemical analyses of Salix alba bark extract were conducted, and nanoparticles of the same extract were synthesized and characterized using UV-vis spectroscopy, XRD (X-Ray Diffraction), SEM (Scanning Electron Microscopy) and EDX (Energy Dispersive X-ray Spectroscopy) techniques.

Results: The qualitative phytochemical analysis of Salix alba bark extract revealed the presence of phenolics, flavonoids, reducing sugars, and saponins. When applied on juveniles of the plant parasitic nematode Meloidogyne incognita, the nanoparticles demonstrated a dose and time-dependent impact. After 24 hours, the highest concentration (1000 µg/ml) of nanoparticles exhibited the greatest mortality, reaching 82.2%, while the lowest concentration resulted in a mortality rate of 34.5%. Similarly, at the 48-hour mark, the highest mortality (92.2%) was observed with 1000 µg/ml of nanoparticles, whereas the lowest concentration yielded a mortality rate of 54.5%. Extending the observation period to 72 hours, the mortality rate peaked at 98.33% with the highest nanoparticle concentration (1000 µg/ml), and the lowest mortality rate was recorded at the lowest dose, amounting to 72.5%. These results underscore the dose and time-dependent efficacy of Salix alba bark-derived nanoparticles against Meloidogyne incognita.

Conclusion: Our findings suggest that zinc oxide nanoparticles synthesized from Salix alba bark can be an effective agent against plant parasitic nematode Meloidogyne incognita. Further experiments are recommended to study the impact of these nanoparticles on other biotic stresses.


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References


Hussain M, Mukhtar T, Kayani MJJAPS. Assessment of the damage caused by Meloidogyne incognita on okra (Abelmoschus esculentus). The Journal of Animal & Plant Sciences, (2011); 21(4): 857-861.

Brand D, Roussos S, Pandey A, Zilioli PC, Pohl J, et al. Development of a bionematicide with Paecilomyces lilacinus to control Meloidogyne incognita. Applied biochemistry and biotechnology, (2004); 11881-88.

Khan A, Ali Khan A, Jameel M, Farhan Khan M, Khan M, et al. Grass-Shaped Zinc Oxide Nanoparticles Synthesized by the Sol-Gel Process and Their Antagonistic Properties towards the Biotrophic Parasite, Meloidogyne incognita. Bioinorganic Chemistry and Applications, (2023); ID 6834710.

Fabiyi OAJV. Sustainable management of Meloidogyne incognita infecting carrot (Daucus carota): green synthesis of silver nanoparticles with Cnidoscolus aconitifolius. Vegetos, (2021); 34(2): 277-285.

Hussain A, Yasar M, Ahmad G, Ijaz M, Aziz A, et al. Synthesis, characterization, and applications of iron oxide nanoparticles. Nanotechnology, science and applications, (2023); 17(4): 3.

Kalaiselvi D, Sundararaj P, Premasudha P, Hafez SLJIJoN. Nematicidal activity of green synthesized silver nanoparticles using plant extracts against root-knot nematode Meloidogyne incognita. International Journal of Nematology, (2017); 27(1): 81-94.

Nour El-Deen AH, El-Deeb BAJJoAS. Effectiveness of silver nanoparticles against root-knot nematode, Meloidogyne incognita infecting tomato under greenhouse conditions. Journal of Agricultural Science, (2018); 10(2): 148.

Gunalan S, Sivaraj R, Rajendran VJPiNSMI. Green synthesized ZnO nanoparticles against bacterial and fungal pathogens. Progress in Natural Science: Materials, (2012); 22(6): 693-700.

El-Deen A, El-Deeb BA. Effectiveness of silver nanoparticles against root-knot nematode, Meloidogyne incognita infecting tomato under greenhouse conditions. J Agric Sci, (2018); 10148-156.

Ahmad S, Ahmad N, Ullah S, Rauf S, Malik S, et al. Advances in metal oxide nanoparticles for cancer therapy and their potential in combating COVID-19. Bioscience Research, (2023).

Ealia SAM, Saravanakumar MP. A review on the classification, characterisation, synthesis of nanoparticles and their application; 2017. IOP conference series: materials science and engineering, pp. 032019.

Nadaroglu H, GÜNGÖR AA, Selvi İJIJoIR, Reviews. Synthesis of nanoparticles by green synthesis method. International Journal of Innovative Research and Reviews, (2017); 1(1): 6-9.

Khan A, Ahmad G, Ijaz M, Ahsan K, Akhtar H, et al. Prevalence and Antibiotic Resistance of Salmonella Species Isolated from Chicken Liver in Peshawar. J Clin Med Res, (2021); 3(3): 1-11.

Singh J, Dutta T, Kim K-H, Rawat M, Samddar P, et al. ‘Green’synthesis of metals and their oxide nanoparticles: applications for environmental remediation. Journal of nanobiotechnology, (2018); 16(1): 1-24.

Fowsiya J, Madhumitha G, Al-Dhabi NA, Arasu MVJJoP, Biology PB. Photocatalytic degradation of Congo red using Carissa edulis extract capped zinc oxide nanoparticles. Journal of Photochemistry and Photobiology B: Biology, (2016); 162395-401.

Pan SY, Litscher G, Gao SH, Zhou -F, Yu ZL, et al. Historical perspective of traditional indigenous medical practices: the current renaissance and conservation of herbal resources. Evidence-based complementary and alternative medicine, (2014); Article ID 525340.

Ullah S, Ullah A, Rahman W, Ullah F, Khan SB, et al. An innovative user-friendly platform for Covid-19 pandemic databases and resources. Comput Methods Programs Biomed Update, (2021); 1100031.

Zabihi NA, Mahmoudabady M, Soukhtanloo M, Hayatdavoudi P, Beheshti F, et al. Salix alba attenuated oxidative stress in the heart and kidney of hypercholesterolemic rabbits. Avicenna Journal of Phytomedicine, (2018); 8(1): 63.

Shara M, Stohs SJJPR. Efficacy and safety of white willow bark (Salix alba) extracts. Phytotherapy Research, (2015); 29(8): 1112-1116.

Ullah S, Rahman W, Ullah F, Ullah A, Ahmad G, et al. AVPCD: a plant-derived medicine database of antiviral phytochemicals for cancer, Covid-19, malaria and HIV. Database (Oxford), (2023); 2023.

Nahrstedt A, Schmidt M, Jäggi R, Metz J, Khayyal MTJWWMW. Willow bark extract: the contribution of polyphenols to the overall effect. WMW Wiener Medizinische Wochenschrift, (2007); 157(13): 348-351.

Islam NU, Jalil K, Shahid M, Rauf A, Muhammad N, et al. Green synthesis and biological activities of gold nanoparticles functionalized with Salix alba. Arabian Journal of Chemistry, (2019); 12(8): 2914-2925.

Ijaz M, Ahmad G, Anjum F, Zeb U, Muhammad N, et al. Morphological Identification and Resistance Profile of Antibiotic and Heavy Metals-Resistant Bacteria in Hospital Sewage of Peshawar. Advancements in Life Sciences, (2023); 10(3): 452-456.

Hussain T, Usman S, Khan A, Hussain A, Aziz A, et al. Isolation and Characterization of Salt Tolerant Bacteria from Saline Areas of Khyber Pakhtunkhwa. Journal of Clinical and Medical Research, (2022); 4(4): 1-8.

Majeed S, Khanday MJOJoC. Green synthesis of silver nanoparticles using bark extract of Salix alba and its antimicrobial effect against bacteria isolated from dental plaque. Oriental Journal of Chemistry, (2016); 32(3): 1611-1618.

Thema F, Manikandan E, Dhlamini M, Maaza MJML. Green synthesis of ZnO nanoparticles via Agathosma betulina natural extract. Biochem anal biochem, (2015); 161124-127.

Wadood A, Ghufran M, Jamal SB, Naeem M, Khan A, et al. Phytochemical analysis of medicinal plants occurring in local area of Mardan. Biochem anal biochem, (2013); 2(4): 1-4.

Krishnamoorthy V, Nagappan P, Sereen AK, Rajendran RJIJCMAS. Preliminary phytochemical screening of the fruit rind of Garcinia cambogia and leaves of Bauhinia variegate-A comparative study. Int J Multidisc Appr Stud, (2014); 3(5): 479-486.

Uddin G, Rauf AJM-Ejompr. Phytochemical screening, antimicrobial and antioxidant activities of aerial parts of Quercus robur L. Middle-East journal of medicinal plants research, (2012); 1(1): 1-4.

Ullah R, Bakht J, Shah MRJPJoPS. Green synthesis and characterization of silver nano-particles from pharmaceutically important Periploca hydaspidis and their biological activity. Pakistan Journal of Pharmaceutical Sciences, (2018); 31(4): 1267-1277.

Rahim A, Ahmad G, Ijaz M, Akhtar H, Ahsan K, et al. Isolation and Identification of Antibiotics Susceptible Staph aureus from Unprocessed Milk. Journal of Clinical and Medical Research, (2021); 3(2): 1-11.

Nuspl M, Wegscheider W, Angeli J, Posch W, Mayr MJA, et al. Qualitative and quantitative determination of micro-inclusions by automated SEM/EDX analysis. Analytical and bioanalytical chemistry, (2004); 379640-645.

El-Batal AI, Attia MS, Nofel MM, El-Sayyad GSJJoCS. Potential nematicidal properties of silver boron nanoparticles: synthesis, characterization, in vitro and in vivo root-knot nematode (Meloidogyne incognita) treatments. Journal of Cluster Science, (2019); 30687-705.

Zhao Y, Wang J, Kuang D, Xu J, Yang M, et al. Susceptibility of tree shrew to SARS-CoV-2 infection. Scientific reports, (2020); 10(1): 16007.

Ahmad H, Venugopal K, Rajagopal K, De Britto S, Nandini B, et al. Green synthesis and characterization of zinc oxide nanoparticles using Eucalyptus globules and their fungicidal ability against pathogenic fungi of apple orchards. Biomolecules, (2020); 10(3): 425.

Basnet P, Chanu TI, Samanta D, Chatterjee SJJoP, Biology PB. A review on bio-synthesized zinc oxide nanoparticles using plant extracts as reductants and stabilizing agents. Journal of Photochemistry and Photobiology B: Biology, (2018); 183201-221.

Barzinjy AA, Azeez HHJSAS. Green synthesis and characterization of zinc oxide nanoparticles using Eucalyptus globulus Labill. leaf extract and zinc nitrate hexahydrate salt. SN Applied Sciences, (2020); 2(5): 991.

Nagarajan S, Arumugam Kuppusamy KJJon. Extracellular synthesis of zinc oxide nanoparticle using seaweeds of gulf of Mannar, India. Journal of nanobiotechnology, (2013); 111-11.

Mahamuni PP, Patil PM, Dhanavade MJ, Badiger MV, Shadija PG, et al. Synthesis and characterization of zinc oxide nanoparticles by using polyol chemistry for their antimicrobial and antibiofilm activity. Biochemistry and biophysics reports, (2019); 1771-80.

Selim YA, Azb MA, Ragab I, HM Abd El-Azim MJSr. Green synthesis of zinc oxide nanoparticles using aqueous extract of Deverra tortuosa and their cytotoxic activities. Scientific reports, (2020); 10(1): 3445.

Pillai AM, Sivasankarapillai VS, Rahdar A, Joseph J, Sadeghfar F, et al. Green synthesis and characterization of zinc oxide nanoparticles with antibacterial and antifungal activity. Journal of Molecular Structure, (2020); 1211128107.

Salam HA, Sivaraj R, Venckatesh RJMl. Green synthesis and characterization of zinc oxide nanoparticles from Ocimum basilicum L. var. purpurascens Benth.-Lamiaceae leaf extract. Materials letters, (2014); 13116-18.

Al Banna LS, Salem NM, Jaleel GA, Awwad AM. Green synthesis of sulfur nanoparticles using Rosmarinus officinalis leaves extract and nematicidal activity against Meloidogyne javanica. Chemistry International, (2020); 6(3): 137-143.

Sikandar A, Zhang M, Wang Y, Zhu X, Liu X, et al. In vitro evaluation of Penicillium chrysogenum Snef1216 against Meloidogyne incognita (root-knot nematode). Scientific reports, (2020); 10(1): 1-9.




DOI: http://dx.doi.org/10.62940/als.v10i4.1581

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