Serum Anti-Nuclear Antibodies and their patterns on immunofluorescence in patients with joint pain from Lahore, Pakistan

Muhammad Daood Khan, Bushra Mubarak, Kainat Waheed, Iqra Munir

Abstract


Background: Autoimmunity is the deviation of the immune system from its natural course and evoking a potent inflammatory response against organs or systems of its own body. Anti-nuclear antibodies (ANA) are an important marker for the diagnosis of autoimmune. The incidence of autoimmune diseases is increasing globally due to advancements in civilization, so it becomes crucial to monitor its trends. The current study aimed to determine the prevalence of ANA and its specific pattern in individuals with joint pain.

Methods: The duration of this cross-sectional study was 6 months, from December 2018 to May 2019. ANA profiles of 155 subjects were evaluated using the indirect immunofluorescence (IIF) technique.

Results: Amongst 155 enrolled subjects 101(65.2%) were females and 54(34.8%) were males. Rheumatoid factor was positive in 61 (39.3%) patients. ANA was found positive in 35 (22.5%) individuals, 5(14.3%) males, and 30(85.7%) females. Homogenous pattern was positive in 25(71.4%), 5(14.3%) fine speckled, 5(14.3%) centromere, and 1(2.8%) presented nuclear dots. The most frequent titer observed was 1/320 in 14(40%) individuals.

Conclusion: The homogenous pattern was frequently observed patterns ANA positive subjects. There was also a significant relationship between age group and their patterns. The homogenous pattern was most frequent among the 60-69-year age group.


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References


Theofilopoulos AN, Kono DH, Baccala R. The multiple pathways to autoimmunity. Nature immunology, (2017); 18(7): 716-724.

Klein L, Kyewski B, Allen PM, Hogquist KA. Positive and negative selection of the T cell repertoire: what thymocytes see (and don't see). Nature Reviews Immunology, (2014); 14(6): 377-391.

Rosenblum MD, Remedios KA, Abbas AK. Mechanisms of human autoimmunity. The Journal of clinical investigation, (2015); 125(6): 2228-2233.

Vojdani A. A potential link between environmental triggers and autoimmunity. Autoimmune diseases, (2014); 20(14): 283-291.

Bach J-F. The hygiene hypothesis in autoimmunity: the role of pathogens and commensals. Nature Reviews Immunology, (2018); 18(2): 105-120.

Jörg S, Grohme DA, Erzler M, Binsfeld M, Haghikia A, et al. Environmental factors in autoimmune diseases and their role in multiple sclerosis. Cellular and Molecular Life Sciences, (2016); 73(24): 4611-4622.

Manzel A, Muller DN, Hafler DA, Erdman SE, Linker RA, et al. Role of “Western diet” in inflammatory autoimmune diseases. Current allergy and asthma reports, (2014); 14(1): 1-8.

Pisetsky DS. Antinuclear antibody testing—misunderstood or misbegotten? Nature Reviews Rheumatology, (2017); 13(8): 495-502.

Grygiel-Górniak B, Rogacka N, Puszczewicz M. Antinuclear antibodies in healthy people and non-rheumatic diseases–diagnostic and clinical implications. Reumatologia/Rheumatology, (2018); 56(4): 243-248.

Faderl M, Klein F, Wirz OF, Heiler S, Albertí-Servera L, et al. Two distinct pathways in mice generate antinuclear antigen-reactive B cell repertoires. Frontiers in immunology, (2018); 9(16): 137-151.

Satoh M, Chan EK, Ho LA, Rose KM, Parks CG, et al. Prevalence and sociodemographic correlates of antinuclear antibodies in the United

States. Arthritis & Rheumatism, (2012); 64(7): 2319-2327.

Mengeloglu Z, Tas T, Kocoglu E, Aktas G, Karabörk S. Determination of anti-nuclear antibody pattern distribution and clinical relationship. Pakistan journal of medical sciences, (2014); 30(2): 380-386.

Konstantinov KN, Rubin RL. The universe of ANA testing: a case for point-of-care ANA testing. Autoimmunity Highlights, (2017); 8(1): 1-4.

Kumar Y, Bhatia A, Minz RW. Antinuclear antibodies and their detection methods in diagnosis of connective tissue diseases: a journey

revisited. Diagnostic pathology, (2009); 4(1): 1-10.

Sakthiswary R, Rajalingam S, Norazman MR, Hussein H. Antinuclear antibodies in primary osteoarthritis of the knee: a case-control study. EXCLI journal, (2012); 7(6): 11624-11631.

Wang K-Y, Yang Y-H, Chuang Y-H, Chan P-J, Yu H-H, et al. The initial manifestations and final diagnosis of patients with high and low titers of antinuclear antibodies after 6 months of follow-up. Journal of Microbiology, Immunology and Infection, (2011); 44(3): 222-228.

Mariz HA, Sato EI, Barbosa SH, Rodrigues SH, Dellavance A, et al. Pattern on the antinuclear antibody–HEp‐2 test is a critical parameter for discriminating antinuclear antibody–positive healthy individuals and patients with autoimmune rheumatic diseases. Arthritis & Rheumatism, (2011); 63(1): 191-200.

Frodlund M, Dahlström Ö, Kastbom A, Skogh T, Sjöwall C. Associations between antinuclear antibody staining patterns and clinical features of systemic lupus erythematosus: analysis of a regional Swedish register. BMJ open, (2013); 3(10): e003608.

Marin GG, Cardiel MH, Cornejo H, Viveros ME. Prevalence of antinuclear antibodies in 3 groups of healthy individuals: blood donors, hospital personnel, and relatives of patients with autoimmune diseases. JCR: Journal of Clinical Rheumatology, (2009); 15(7): 325-329.

Sharmin S, Ahmed S, Saleh AA, Rahman F, Choudhury M, et al. Association of immunofluorescence pattern of antinuclear antibody with specific autoantibodies in the Bangladeshi population. Bangladesh Medical Research Council Bulletin, (2014); 40(2): 74-78.

Racoubian E, Zubaid RM, Shareef MA, Almawi WY. Prevalence of antinuclear antibodies in healthy Lebanese subjects, 2008–2015: a cross-sectional study involving 10,814 subjects. Rheumatology international, (2016); 36(9): 1231-1236.

Al-Mughales JA. Anti-Nuclear Antibodies Patterns in Patients With Systemic Lupus Erythematosus and Their Correlation With Other Diagnostic Immunological Parameters. Frontiers in Immunology, (2022); 13(2): 159-165.

Nanda R, Gupta P, Patel S, Shah S, Mohapatra E. Uncommon antinuclear antibody patterns as diagnostic indicators. Clinical Biochemistry,

(2021); 17(1): 9028-33.

Hayashi N, Uto K, Imanishi A, Sugiyama D, Morinobu A, et al. Prevalence of anti-dense fine speckled 70 antibodies in healthy individuals and patients with antinuclear antibody-associated autoimmune rheumatic diseases in Japan. Medicine, (2021); 100(9): 219-234.

Wei Q, Jiang Y, Xie J, Lv Q, Xie Y, et al. Analysis of antinuclear antibody titers and patterns by using HEp‐2 and primate liver tissue substrate indirect immunofluorescence assay in patients with systemic autoimmune rheumatic diseases. Journal of Clinical Laboratory Analysis, (2020); 34(12): e23546.




DOI: http://dx.doi.org/10.62940/als.v9i3.1471

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