The effect of Syzygium aromaticum (clove) on inflammatory markers (total leukocyte count, differential leukocyte count and tumor necrosis factor-alpha)

Saima Tabassum, Ambreen Anjum, Sobia Manzoor, Wardah Anwar, Muhammad Hashim Ghouri

Abstract


Background: Inflammation is involved in pathogenesis of many diseases. Antiinflammatory chemicals can be used to treat such illness,especially if they are derived from plant sources as they will have fewer side effects.

Objective:to find out  the outcome of ethanolic extract of syzygium aromaticum flower buds on markers of inflammation in albino rats.

Material and method: Antiinflammatory activity was investigated in albino rats using a experimental model of  inflammation, the “formalin test” (injecting 5 % formalin into subplantar surface of right hind paw). Ninety rats were uniformly divided into three groups designates as control A,experimental B and refrence C. Intraperitoneal  injection of normal saline, syzygium aromaticum flower bud extract and  indomethacin was used. In these rats, foot edema was measured by Vernier caliper. Blood sampling was done through cardiac punture under anaesthesia to determine total and differential leukocyte counts and serum tumor necrosis factor alpha (TNF-α) levels.

 Results: The clove extract  produced significant (p=0.00) decrease  in inflammatory response initiated by 5% formalin. The extract significantly decreased (p=0.009) serum TNF-α. However, its effect on the total and differential leukocyte counts was non-significant (p˃0.05).

Conclusion: The ethanolic extract of syzygium aromaticum  possesses potent  anti-inflammatory effects.


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References


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DOI: http://dx.doi.org/10.62940/als.v9i2.1283

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